We describe a new species of Hypsiboas from the slopes and summit of Auyán-tepui in Canaima National Park, Bolívar State, Venezuela. The new species resembles H. sibleszi, but it differs from that species in having pale green dorsal coloration with numerous small chromatophores, dorsolateral white stripes that extend from the tip of snout to the groin when present, pale interorbital bar absent, a transparent yellow venter, yellow-colored digits and webbing, a more extensive webbing on hands and feet, a prepollical spine not protunding, very narrow nasals partially fused with sphenethmoid, a moderate-sized frontoparietal fontanelle, and an advertisement call consisting of three or four notes with a dominant frequency between 3010 and 3488 Hz. New data on the vocalization, osteology and distribution of H. sibleszi are provided.